cd44 antibody, anti-human, reafinity (Miltenyi Biotec)

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Miltenyi Biotec cd44 antibody, anti-human, reafinity
Cd44 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd44/CD44+Antibody%2C+anti-human%2C+REAfinity/custom%40130-126-473%4042502392
Average 95 stars, based on 80 article reviews

cd44 antibody, anti-human, reafinity - by Bioz Stars, 2026-09

95/100 stars

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Isolation:

Article Title: A novel chalcone analog inhibits cancer stemness in CD44 + and CD133 + populations via suppression of ERK signaling.
Article Snippet: The spheroid culture medium consisted of Advanced DMEM/F12, supplemented with B27 (1×; Cat. No. 17504044, Gibco, Thermo Fisher Scientific, Waltham, MA, USA), N2 (1×, Cat. No. 17502048, Gibco), epidermal growth factor (EGF, 10 ng/ mL; Cat. No. 17502048, Gibco), and basic fibroblast growth factor (bFGF, 10 ng/mL; Cat. No. GF003AF, Sigma-Aldrich, St. Louis, MO, USA). .. CD44+ or CD133+ spheroid populations were isolated from dissociated tumor spheroids using anti-CD44 MicroBeads (Cat. No.130-095194, Miltenyi Biotec, Bergisch Gladbach, Germany) or anti-CD133 MicroBeads (Cat. No. 130-097-049, Miltenyi Biotec), respectively, according to the manufacturer's instructions. .. Briefly, tumor spheroids were enzymatically dissociated into single-cell suspensions using Accutase (Cat. No. A1110501; Life Technologies, Thermo Fisher Scientific) and incubated with anti-CD44 or anti-CD133 magnetic beads at 4 ◦C for 15 min.

Incubation:

Article Title: Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia.
Article Snippet: VMSCs and ADSCs were blocked with a human FcR blocking reagent (Miltenyi Biotec), then centrifuged at 500 g at 4°C for 5 min. .. The cells were incubated with allophycocyanin (APC)conjugated antibodies against CD29, CD34, CD44, CD45, CD73, CD90, CD105, and CD141 (Miltenyi Biotec). ..

Article Title: Adipose-derived dual cell therapy enhances arteriogenesis and limb preservation through vascular integration in critical limb ischemia
Article Snippet: VMSCs and ADSCs were blocked with a human FcR blocking reagent (Miltenyi Biotec), then centrifuged at 500 g at 4 °C for 5 min. .. The cells were incubated with allophycocyanin (APC)-conjugated antibodies against CD29, CD34, CD44, CD45, CD73, CD90, CD105, and CD141 (Miltenyi Biotec). ..

Article Title: HERC1 oncogene enhances stemness and tumorigenic potential in CD44 + -derived organoids of head and neck squamous cell carcinoma through IL-6/STAT3 signaling.
Article Snippet: Organoid numbers and sizes were quantified using the ImageJ software (NIH) [34]. .. WS1 fibroblasts and spheroids were fixed with 4% paraformaldehyde for 15min, permeabilized with 0.1% Triton X-100 in PBS for 10min. and blocked with 1% BSA in PBS for 1 h. Cells were incubated overnight at 4 °C with CD44 (FITC-conjugated; Cat. 130-113-334, Miltenyi Biotec), Sox-2 (Cat. 23064, cell Signaling), Slug (Cat. 9585, Cell Signaling), or FAP (Cat. sc-65398, Santa Cruz) antibodies, followed by Alexa Fluor 488/594/647–conjugated secondary antibodies (Cat. A-11008; Cat. A32744; Cat. A32728TR, Life Technologies). .. Nuclei were counterstained with DAPI (Cat. F6057, Sigma), and images were acquired using a Zeiss confocal microscope and processed with Imaris 7.6.

Single Cell:

Article Title: SPRED2 suppresses the stemness of hepatocellular carcinoma through the p53/miR-506-3p/KLF4 pathway.
Article Snippet: .. CD44 and CD90 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) were used to isolate CD44+ and CD90+ cells, respectively, from single cell suspensions of HCC cells. ..

Control:

Article Title: Good Manufacturing Practice-Derived Human Liver Stem Cell Extracellular Vesicles Attenuate Liver Fibrosis In Vivo
Article Snippet: .. The control was carried out on a representative sample of harvested cells post-starvation, analyzing the expression of characteristic surface antigens and markers, such as CD29, CD73, CD105, CD90, CD44, CD14, CD45, CD34 (all from Miltenyi Biotec), and cytoplasmatic albumin (LSBio, Newark, CA, USA). .. The cytofluorimetric analyses were performed using the Miltenyi Flow Cytometer MACSQuant10 and analyzed with the MACSQuantify software version 2.13 with the 21 CFR Part 11 module, specifically designed to support GMP-compliant workflows.

Expressing:

Staining:

Article Title: Targeting stroma-mediated T-cell exclusion and functional exhaustion in pancreatic ductal adenocarcinoma through CXCR4 and PD-1 blockade
Article Snippet: .. Cancer cell were stained for vimentin (#sc-6260, Santa Cruz Biotechnology, Dallas, TX, USA), pancytokeratin (#130-133-439, Miltenyi Biotec), cytokeratin 7 (#130- 115-446, Miltenyi Biotec), E-cadherin (#130-111-992, Miltenyi Biotec), CD44 (#130-113-900, Miltenyi Biotec), Epcam (#14-9326- 82, Thermo Fisher), Ki67 (#MAB7617, R&D Systems, Minnneapolis, MN, USA), and N-cadherin (#ab98952, Abcam, Cambridge, England). .. Patient-derived CAF were immunofluorescence labeled for aSMA (#19245S, Cell Signaling, Danvers, MA, USA).

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